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(A) Human epidermal fibroblasts and <t>keratinocytes</t> were treated with vehicle control (PBS) or GFP mRNA encapsulated by cationic lipid nanoparticle DOTAP or mixed with commercial transfection reagents (Lipofectamine MAX or jetMESSENGER) to assess transfection efficiency. Representative images of green fluorescence from live cells (left panel) and DAPI-stained nuclei are shown ( n = 3 independent experiments). Quantification GFP+ cells/DAPI are presented as mean percentage ± SD ( n = 3). (B) Schematic outline of the experimental setup. (C–E) Representative flow cytometry plots and quantitative analysis of early and late apoptosis in human epidermal keratinocytes, assessed 24 h after ionizing radiation (5 Gy) or no irradiation (No IR control). (F and G) Representative flow cytometry plots and quantitative analysis of Annexin V-positive cells in indicated groups of human epidermal keratinocytes. (H and I) Representative flow cytometry plots and quantitative analysis of Annexin V-positive cells in human dermal microvascular endothelial cells treated with indicated groups. Primary cells were transfected with 1 μg/mL DOTAP LNP encapsulated GFP or TERT mRNA or treated with vehicle control (PBS). Flow cytometry analysis was performed 24 h following 5 Gy irradiation or No IR control. Early apoptosis was identified as Zombie Aqua (−)/Annexin V (+), while late apoptosis was identified as Zombie Aqua (+)/Annexin V (+). Data are shown as mean ± SD ( n = 3). ns, p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. p values were calculated using two-way ANOVA. KTNs, epidermal keratinocytes; MVECs, dermal microvascular endothelial cells; US, unstained.
Keratinocyte Growth Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
keratinocyte growth medium - by Bioz Stars, 2026-02
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dermal cell basal medium plus keratinocyte growth kit  (ATCC)
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(A) Human epidermal fibroblasts and <t>keratinocytes</t> were treated with vehicle control (PBS) or GFP mRNA encapsulated by cationic lipid nanoparticle DOTAP or mixed with commercial transfection reagents (Lipofectamine MAX or jetMESSENGER) to assess transfection efficiency. Representative images of green fluorescence from live cells (left panel) and DAPI-stained nuclei are shown ( n = 3 independent experiments). Quantification GFP+ cells/DAPI are presented as mean percentage ± SD ( n = 3). (B) Schematic outline of the experimental setup. (C–E) Representative flow cytometry plots and quantitative analysis of early and late apoptosis in human epidermal keratinocytes, assessed 24 h after ionizing radiation (5 Gy) or no irradiation (No IR control). (F and G) Representative flow cytometry plots and quantitative analysis of Annexin V-positive cells in indicated groups of human epidermal keratinocytes. (H and I) Representative flow cytometry plots and quantitative analysis of Annexin V-positive cells in human dermal microvascular endothelial cells treated with indicated groups. Primary cells were transfected with 1 μg/mL DOTAP LNP encapsulated GFP or TERT mRNA or treated with vehicle control (PBS). Flow cytometry analysis was performed 24 h following 5 Gy irradiation or No IR control. Early apoptosis was identified as Zombie Aqua (−)/Annexin V (+), while late apoptosis was identified as Zombie Aqua (+)/Annexin V (+). Data are shown as mean ± SD ( n = 3). ns, p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. p values were calculated using two-way ANOVA. KTNs, epidermal keratinocytes; MVECs, dermal microvascular endothelial cells; US, unstained.
Dermal Cell Basal Medium Plus Keratinocyte Growth Kit, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dermal cell basal medium plus keratinocyte growth kit/product/ATCC
Average 97 stars, based on 1 article reviews
dermal cell basal medium plus keratinocyte growth kit - by Bioz Stars, 2026-02
97/100 stars
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(A) Human epidermal fibroblasts and keratinocytes were treated with vehicle control (PBS) or GFP mRNA encapsulated by cationic lipid nanoparticle DOTAP or mixed with commercial transfection reagents (Lipofectamine MAX or jetMESSENGER) to assess transfection efficiency. Representative images of green fluorescence from live cells (left panel) and DAPI-stained nuclei are shown ( n = 3 independent experiments). Quantification GFP+ cells/DAPI are presented as mean percentage ± SD ( n = 3). (B) Schematic outline of the experimental setup. (C–E) Representative flow cytometry plots and quantitative analysis of early and late apoptosis in human epidermal keratinocytes, assessed 24 h after ionizing radiation (5 Gy) or no irradiation (No IR control). (F and G) Representative flow cytometry plots and quantitative analysis of Annexin V-positive cells in indicated groups of human epidermal keratinocytes. (H and I) Representative flow cytometry plots and quantitative analysis of Annexin V-positive cells in human dermal microvascular endothelial cells treated with indicated groups. Primary cells were transfected with 1 μg/mL DOTAP LNP encapsulated GFP or TERT mRNA or treated with vehicle control (PBS). Flow cytometry analysis was performed 24 h following 5 Gy irradiation or No IR control. Early apoptosis was identified as Zombie Aqua (−)/Annexin V (+), while late apoptosis was identified as Zombie Aqua (+)/Annexin V (+). Data are shown as mean ± SD ( n = 3). ns, p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. p values were calculated using two-way ANOVA. KTNs, epidermal keratinocytes; MVECs, dermal microvascular endothelial cells; US, unstained.

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Telomerase mRNA therapy protects human skin against radiation-induced DNA damage

doi: 10.1016/j.ymthe.2025.09.029

Figure Lengend Snippet: (A) Human epidermal fibroblasts and keratinocytes were treated with vehicle control (PBS) or GFP mRNA encapsulated by cationic lipid nanoparticle DOTAP or mixed with commercial transfection reagents (Lipofectamine MAX or jetMESSENGER) to assess transfection efficiency. Representative images of green fluorescence from live cells (left panel) and DAPI-stained nuclei are shown ( n = 3 independent experiments). Quantification GFP+ cells/DAPI are presented as mean percentage ± SD ( n = 3). (B) Schematic outline of the experimental setup. (C–E) Representative flow cytometry plots and quantitative analysis of early and late apoptosis in human epidermal keratinocytes, assessed 24 h after ionizing radiation (5 Gy) or no irradiation (No IR control). (F and G) Representative flow cytometry plots and quantitative analysis of Annexin V-positive cells in indicated groups of human epidermal keratinocytes. (H and I) Representative flow cytometry plots and quantitative analysis of Annexin V-positive cells in human dermal microvascular endothelial cells treated with indicated groups. Primary cells were transfected with 1 μg/mL DOTAP LNP encapsulated GFP or TERT mRNA or treated with vehicle control (PBS). Flow cytometry analysis was performed 24 h following 5 Gy irradiation or No IR control. Early apoptosis was identified as Zombie Aqua (−)/Annexin V (+), while late apoptosis was identified as Zombie Aqua (+)/Annexin V (+). Data are shown as mean ± SD ( n = 3). ns, p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. p values were calculated using two-way ANOVA. KTNs, epidermal keratinocytes; MVECs, dermal microvascular endothelial cells; US, unstained.

Article Snippet: Skin explants were cultured in keratinocyte growth medium (ATCC, no. PCS-200–040) at 37°C, 5% CO 2 , maintaining an air-liquid interphase as described.

Techniques: Control, Transfection, Fluorescence, Staining, Flow Cytometry, Irradiation